Munguva yekuvhara, puroteni inonamatira kune physiologically active BNP (77th kusvika 108th amino acids) uye n-terminal fragment NT-proBNP (1st kusvika 76th amino acids).Apo BNP, iyo 32 amino acids yakareba, yakavharirwa muropa, inosunga kune zvinyorwa zvayo (NPRA neNPRB) uye inogadzirisa ropa kuburikidza nenzira dzakasiyana-siyana.NT-proBNP, iyo 76 amino acids yakareba, haina basa rehupenyu, asi nokuti ine hafu yehupenyu hurefu kupfuura BNP, inonyanya kukodzera sechiratidzo chekuona zvirwere zvakasiyana-siyana zvemwoyo.Mukuongorora kwekliniki yemhuka dzinovaraidza, huwandu hweropa hweNT-proBNP mumbwa hunopfuura 900 pmol / L, uye katsi dzakakwirira kudarika 270 pmol / L, uye kune ngozi yakawanda yezvirwere zvinokonzerwa nemoyo.Zvisinei, zvinofanira kucherechedzwa kuti nokuti NT-proBNP yakabudiswa neitsvo, apo mhuka inotambura nezvirwere zvine chokuita neitsvo, kukanganiswa kweNT-proBNP mumuviri kuchawedzerawo uye nhema dzenhema dzichaonekwa pamuedzo.
Ichi chigadzirwa chinotora fluorescence immunochromatography kuona zvirimo mufNT-proBNP muserum/plasma kuwanda.Nheyo yakakosha: Kune mitsara yeT neC pane nitric acid fiber membrane zvakateerana, uye mutsetse weT wakaputirwa neantibody a inonyatsoziva fNT-proBNP.Iyo musanganiswa pad inopfapfaidzwa neimwe fluorescent nanomaterial yakanyorwa antibody b inogona kunyatso ratidza FDT-probNP.Mumuenzaniso, FDT-probNP inotanga kusanganisa nenanomaterial yakanyorwa antibody b kuti iite yakaoma, uyezve kune yepamusoro chromatography, yakaoma inosanganiswa neT-line antibody A kugadzira sandwich chimiro.Kana iko kunakidzwa kwemwenje wemwenje, iyo nanomaterial inoburitsa masaini efluorescence.Kusimba kwechiratidzo kwakanyatso kuenderana nekusangana kwefNT-proBNP mumuenzaniso.
Kubva payakavambwa, fekitori yedu yanga ichigadzira zvigadzirwa zvepasi rose nekutevedzera musimboti
zvemhando yekutanga.Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindastiri uye kuvimbika kwakakosha pakati pevatengi vatsva nevakuru..