Canine Cortisol (cCortisol) is steroid hormone inogadzirwa ne canine adrenal cortex.Cortisol inobatsira kuchengetedza huwandu hweshuga yeropa, inoderedza kuzvimba uye inobatsira muviri kugadzirisa kushushikana.Kunyange zvazvo mamiriro ezvinhu akasiyana-siyana anokonzerwa nemazinga akawandisa ehomoni yedoro anonzi Cushing syndrome (CS), zvose imbwa nekatsi zvinogona kutambura neCS, iyo inowanzoonekwa mumbwa pane katsi.Imbwa dzepakati nekukwegura (anenge makore 7 kusvika ku12)
vane mukana mukuru wekuita chirwere.Chirwere ichi chinokura zvishoma nezvishoma uye zviratidzo zvekutanga hazvisi nyore kuona.CS inogona kuongororwa nekliniki ne adrenocorticotropic hormone (ACTH) stimulation test uye dexamethasone suppression test, uye marudzi ayo akasiyana: adrenal-dependent (ATH) uye pituitary-dependent (PDH).
Ichi chigadzirwa chinoshandisa fluorescence immunochromatography kuona zvakanyanya zviri mukati cCortisol mumbwa serum/plasma.Nheyo yekutanga: T uye C mitsetse yakanyorwa pane nitrocellulose membrane, mutsara weT wakaputirwa necCortisol antigen a, uye pedhi yekusungira inopfapfaidzwa nefluorescent nanomaterial yakanyorwa antibody b inogona kunyatso ziva cCortisol.
iye cCortisol mumuenzaniso inotanga kunyorwa nenanomaterial.Masoja ekudzivirira chirwere b anosungirirana kuita yakaoma, uyezve chromatographs kumusoro.Iyo yakaoma inokwikwidza neT-line antigen a uye haigone kutorwa;Zvakasiyana, kana pasina cCortisol mumuenzaniso, antibody b inosunga kune antigen a.Kana chiedza chekusimudzira chikavhenekerwa, iyo nano zvinhu inoburitsa chiratidzo chefluorescent, uye simba rechiratidzo rinopesana nekusangana kwecCortisol mumuenzaniso.
Kubva payakavambwa, fekitori yedu yanga ichigadzira zvigadzirwa zvepasi rose nekutevedzera musimboti
zvemhando yekutanga.Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindastiri uye kuvimbika kwakakosha pakati pevatengi vatsva nevakuru..