New Tech's yakazara otomatiki nucleic acid kudhirowa uye yekuona muchina

Simba shanu :
● Chiridzwa chakagadzirirwa ne nucleic acid kutorwa uye danho rakacheneswa
● Chiridzwa chakagadzirirwa ne ultrasonic extraction module
● Chiridzwa chakagadziridzwa zvine otomatiki zvizere
● Chiridzwa chakagadziridzwa neinochinja tembiricha amplification
● Chiridzwa chakagadziridzwa chine yakavharwa zvizere reagent kit

1. Ko nucleic acid inoona reagents inoda kutorwa nekucheneswa?
Nheyo yekuona nucleic acid ndeyekutevera: pasi pekuita kweprimer, DNA polymerase inoshandiswa kuita chain reaction amplification patemplate DNA/RNA (inoda reverse transcription yeNA), uyezve huwandu hwechiratidzo chefluorescent chakaburitswa chinoonekwa kuti chionekwe. kana sampuli ine nucleic acid (DNA / RNA) yepathogen kuti ionekwe.

1) Mienzaniso isina kubviswa kana kucheneswa inogona kunge iine zvinhu zvakawanda zvinokanganisa chigumisiro chekupedzisira: nuclease (iyo inogona kunyungudutsa chinangwa nucleic acid uye kukonzera nhema dzisina kunaka), protease (iyo inogona kuderedza DNA polymerase uye kukonzera nhema dzisina kunaka), heavy metal. munyu (iyo inotungamirira kusashanda kwe synthase uye inokonzera nhema yakanaka), yakanyanyisa acidic kana yakanyanyisa alkaline PH (iyo inogona kuita kuti mhinduro yacho ikundike), Isina kukwana RNA (inotungamirira kune nhema dzisina kunaka reverse transcription failure).

2) Mimwe sampuli yakaoma kukwidziridza zvakananga: Gram-positive uye mamwe maparasite, nekuda kweawo makobvu maseru madziro uye zvimwe zvimiro, kana akasapfuura neiyo nucleic acid kudhirowa uye kucheneswa maitiro, iyo yekubvisa-yemahara kit inogona kukundikana kune akadaro. samples.

Naizvozvo, zvinokurudzirwa kusarudza kit yekuyedza kana chiridzwa chakagadziriswa neiyo nucleic acid yekubvisa nhanho.

2. Kemikari kudhirowa kana muviri ultrasonic fragmentation extraction?
Kazhinji kutaura, kudhirowa kwemakemikari kunogona kuiswa kune yakawanda ye pretreatment uye Purification.Nekudaro, mugobvu-wakavharwa neGram-positive mabhakitiriya uye mamwe maparasite, zvakare inyaya yekuti kubviswa kwekemikari hakugone kuwana matemplate anoshanda nucleic acid, zvichikonzera kuonekwa kwenhema.Mukuwedzera, kubudiswa kwemakemikari kunowanzoshandisa vamiririri vane simba, kana kusvibiswa kusina kukwana, zviri nyore kuunza alkali yakasimba mukugadzirisa maitiro, zvichiita kuti zvive nemigumisiro isina kururama.

Ultrasonic fragmentation inoshandisa kupwanya kwemuviri, iyo yakashandiswa zvinobudirira neGeneXpert, bhizinesi rinotungamira mumunda wePOCT kuti ishandiswe nevanhu, uye ine mukana wakakwana mukutora nucleic acid yemamwe masampuli akaoma kunzwisisa (akadai seMycobacterium tuberculosis).

Naizvozvo, zvinokurudzirwa kusarudza kit yekuyedza kana chiridzwa chakagadziridzwa ine nucleic acid yekubvisa nhanho.uye ndiyo yakakwana kana paine ultrasonic extraction module.

3. Manual , Semi-otomatiki uye zvizere otomatiki?
Iri idambudziko rekudhura kwevashandi uye kugona kushanda zvakanaka.Parizvino, zvipatara zvezvipfuyo zvisina vashandi vakakwana, uye nucleic acid kutorwa nekuonekwa ibasa rinoda hunyanzvi uye ruzivo.Hapana mubvunzo kuti iyo yakazara otomatiki nucleic acid kudhirowa uye yekuona muchina ndiyo sarudzo yakakwana.

4. Kukwidziridzwa kwetembiricha nguva dzose kana kuwedzera tembiricha?
Iyo amplification reaction ndeye nucleic acid yekuona link, uye tekinoroji yehunyanzvi inobatanidzwa mune iyi link yakaoma.Kutaura zvazviri, ma enzymes anoshandiswa kuwedzera nucleic acid.Mukuita kukwidziridzwa, iyo yakawedzera fluorescence siginecha kana yakadzikwa yefluorescence sigiro inoonekwa.Kazhinji kutaura, iyo yekutanga iyo chiratidzo chefluorescence ichioneka, iyo yakakura iyo inotariswa gene yemukati yemuenzaniso.

Constant temperature amplification is nucleic acid amplification at fixed temperature, ukuwo variable tembiricha amplification iri cyclic amplification zvinoenderana nedenaturation-annealing-extension.Iyo inogara tembiricha yekuwedzera tembiricha yakaitwa, nepo kuchinjika tembiricha yekusimudzira nguva inokanganiswa zvakanyanya nechiyero chekushisa kukwira nekudonha kwechiridzwa (parizvino, vagadziri vazhinji vakakwanisa kuita makumi mana ekutenderera kwemaminetsi makumi matatu).

Kana mamiriro emurabhoritari akanaka uye nzvimbo yacho yakaoma, zvine musoro kutaura kuti mutsauko wechokwadi pakati pezviviri hauzove wakakura.Nekudaro, kusiyanisa tembiricha kukwidziridzwa kuchagadzira akawanda nucleic acid zvigadzirwa munguva ipfupi.Kune marabhoritari asina kuomesesa kuganhurwa uye vashandi vehunyanzvi kudzidzisa, njodzi yenyucleic acid aerosol kuvuza ichave yakakura, iyo yenhema yakanaka inoitika kana kuvuza kwaitika, uye zvinova zvakanyanya kuoma kubvisa.

Uye zvakare, Constant tembiricha kukwidziridzwa kunowedzerawo kune kusiri-chaiyo amplification kana sampuli yakaoma (iyo yehukama tembiricha yakadzikira, uye nepamusoro tembiricha yekuwedzera, zviri nani iyo primer inosunga chirevo).

Nezve tekinoroji yazvino ine chekuita, kusiyanisa tembiricha amplification inovimbika zvakanyanya.

5. Nzira yekudzivirira sei njodzi yekudonha kweiyo nucleic acid amplification zvigadzirwa?
Parizvino, vagadziri vazhinji vanosarudza tembiricha yePCR chubhu seiyo nucleic acid reaction chubhu, yakavharwa nekukweshana, uye tembiricha denaturation mune inoshanduka tembiricha denaturation mukusiyana tembiricha PCR amplification inosvika 90 degree.
Centigrade.Iyo inodzokororwa nzira yekuwedzera nekupisa uye kupesana nekutonhora idambudziko guru pakuvharwa kwePCR chubhu, uye gland rudzi PCR chubhu iri nyore kukonzera kuvuza.

Zviri nani kutora maitiro nekiti / chubhu yakavharwa zvachose kudzivirira kubuda kwechigadzirwa chekuita.Zvingave zvakakwana kana paine basa rekubuda rakavharwa zvizere rekuti nucleic acid ibviswe nekuonekwa.

Saka New Tech's nyowani yakazara otomatiki nucleic acid yekubvisa uye yekuona muchina une sarudzo shanu dziri pamusoro.